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The Division of Biological Sciences Protein Sequencing
Facility is located in Room 2325A in Bonner Hall on the Revelle Campus
of the University of California at San Diego in La Jolla, California (See
map). The Facility operates an Applied Biosystems PROCISE 494HT Protein
Sequenator. San
Diego; UCSD University of California; San Diego protein sequencing,UCSD,
University of California San Diego, San Diego, La Jolla, California, protein
sequencing, protein sequencer, sequencing, sequncer, Edman chemistry,
Edman sequencing, Procise 494 sequencer, ABI 494
- Facility Operator:
Matthew Williamson
- Description of Services:
- Provide Edman sequencing of protein and peptide
samples
- Samples either in solution or transferred from
polyacrylamide gels to PVDF membranes
- Typical requirement is 5 pmol of purified material,
although the absolute sensitivity of the instrument is on the
order of 0.5 pmol - We will gladly attempt lower amounts,
if you are unable to obtain larger quantities
- Samples in solution are usually adsorbed to PVDF
membranes using an Applied Biosystems PROSORB device
- Sequence data screened against available web-based
online databases for identification or sequence homologies
- Charges for Services:
- University of California Users:
- $31/Amino Acid Residue for amino acids 1-10
(minimum charge is 5 residues)
- After 10 amino acids, residues 11 and beyond
are charged half-price
- For Users Outside UC System working at a Non-Profit
Organization :
- $35.50/Amino Acid Residue for amino acids 1-10
(minimum charge is 5 residues)
- After 10 amino acids, residues 11 and beyond
are charged half-price
- For Users Outside UC System working at a 'For-Profit'
Organization :
- $46/Amino Acid Residue for amino acids 1-10
(minimum charge is 5 residues)
- After 10 amino acids, residues 11 and beyond
are charged half-price
- Cost of Adsorbing Sample to PROSORB device: $10/sample
- Requirements for Samples:
- Approximately 85% purity of sample
- Sample in solution or transferred from gel to PVDF
membrane and stained with Coomassie blue, Ponseau S, or Amido black
- Sample Submission Procedure:
- Download Sample Submission
Form, fill it out and send or bring with your sample(s)
- Contact Matt Williamson if you have any questions
(see contact information above)
- Mail sample using Federal Express or bring to lab
in person
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